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The Hassall bodies (HB) were classified as juvenile, immature, mature, senescent, and lymphocyte-rich subtypes according to previous studies [41, 42]. Briefly, juvenile HB were small-sized or medium-sized, ovoid or irregular formations of thymic epithelial cells with unaltered cellular morphology, without prominent cytoplasmic acidophilia, necrosis or cellular debris. Immature HB were round or oval formations, consisting of squamous thymic epithelial cells with cytoplasmic acidophilia, but without degenerative changes. Mature HB were medium- sized or large-sized epithelial formations, with necrotic or cystic degenerative changes in their central area, but presenting squamous thymic epithelial cells this website at their periphery. Senescent HB were large-sized formations, without any morphologically recognizable thymic epithelial cells, but with calcified, necrotic material, cellular debris, or cystic dilatation. Lymphocyte-rich HB were medium- or large-sized formations, containing groups of lymphocytes [41, 42]. The counting of immunopositive cells was performed in all subtypes of HB except for senescent HB [42]. I-BET-762 supplier 2.3. Statistical Analysis Student's t-test was used for statistical analysis. The results were considered as statistically significant when P Fluvoxamine in Thymocytes (Table 1 and Figures ?Figures11 and ?and22) Figure 1 Immunohistological analysis of thymus using the double streptavidin-biotin peroxidase-labeled (brown staining)/alkaline phosphatase and antialkaline phosphatase (red staining) immunohistochemical procedures. Double immunostaining for (a) p-c-Jun (brown)/CD163 ... Figure 2 Immunohistological analysis of thymus using the double streptavidin-biotin peroxidase-labeled (brown staining)/alkaline phosphatase and antialkaline phosphatase (red staining) immunohistochemical procedures. Double immunostaining for (a) p-c-Jun (brown)/CD3 ... Table 1 Expression of p-c-Jun, JunB, JunD, p-STAT3, p-STAT5, and p-STAT6 in thymocytes. p-c-Jun, JunB, p-STAT3, p-STAT5, and p-STAT6 nuclear expression was detected in a few scattered cortical and medullary thymocytes (