Human airway basal cells were infected with lentivirus expressing GFP (Lenti-GFP) or NICD1-4 (Lenti-NICD1-4) and cultured on ALI for 28 days

Relative to control cells (Lenti-GFP), constitutive activation of Notch signaling via expression of NICD2 or NICD4 had no significant effect on regulating differentiation of BC into secretory (5.0% Lenti-GFP vs 5.3% Lenti-NICD2, p>.5 and five.% Lenti-GFP vs 5.2% 1-Pyrrolidinebutanoic acid,��-[3-(3,5-dimethyl-1H-pyrazol-1-yl)phenyl-3-[2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl]-,(��S,3R)- (hydrochloride)] Lenti-NICD4, p>0.five, Fig. Consistent with the histological differentiation data, analysis of the secretory cell markers MCE Chemical 1235449-52-1 MUC5AC and SCGB1A1 revealed significant increased expression (both p