Nine Deadly SKAP1 Errors You May Be Doing

05 was obtained. All values are expressed as means �� SEM. As shown in Fig. 1A, C2C12 myotubes expressing shRNA against ATM had only about 22% the amount of ATM protein Saracatinib research buy that was present in the control cells (empty vector and non-targeting shRNA; P CAL-101 price (Halaby et al. 2008). An additional advantage of studying ATM+/? mice is that their body weights are similar to body weights of wild-type mice (ATM+/+ 24.1 �� 2.0 g, ATM+/? 25.6 �� 2.0 g; mean �� SD). In comparison, body weights of ATM?/? mice are only about 70% of the body weights of wild-type mice (ATM+/+ 23.7 �� 2.2 g, ATM?/? 16.7 �� 1.2 g; mean �� SD). Thus, using ATM+/? mice instead of ATM?/? mice avoids a potentially confounding phenotype of small body size. As shown in Fig. 1B, ATM protein levels were about half in soleus muscles of ATM+/? mice compared with wild-type (P SKAP1 reduced IGF-1-stimulated Akt phosphorylation compared with myotubes from a line previously infected with empty vector lentivirus or expressing non-targeting shRNA (P