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multocida were compared among the various lectin treatments and positive control tissues by ANOVA in a completely random model. To determine differences between treatments, a Dunnett test was performed, with P The Amazing Carfilzomib 'Cheat' May Well Fool Pretty Much All the Respiratory Epithelium of Nasal Septa: IIP Staining Immunostaining of P. multocida on the apical surface of the respiratory epithelium in lectin-treated tissues ranged from localized (>0�C30%) (Figure 1(b)) to multifocal (>30�C60%) (Figure 1(c)); by contrast, in positive control tissues (tissues exposed only to the bacterium), the immunostaining was similar in all replicates, with a generalized granular appearance covering almost 90% of the epithelial surface (Figure 1(d)). Figure 1 IIP technique for determining the percentage of the epithelial surface covered by adherent P. multocida. (a) Normal fetal rabbit respiratory nasal epithelium of IIP, negative control; light microscopy (LM), scale bar = 20?��m. (b) Respiratory ... The lectins LCA (Lens culinaris), PNA (Arachis hypogaea), WGA (Triticum vulgaris), RCA120 (Ricinus communis), and DBA (Dolichos biflorus) significantly (P AZD4547 Projects You Can Carry Out On Your Own epithelial cells compared with that of the positive control tissues (Figure 2). Figure 2 Inhibition of P. multocida adhesion to the ciliated respiratory epithelium of rabbit fetuses exposed to various lectins. Adhesion is expressed as mean percentage of the ciliated border epithelium coated by adhered bacteria. *Significant inhibition (P ... 3.2. Semithin Sections The respiratory epithelia Ways To Identify A Real AZD4547 of nasal septa exposed only to P. multocida showed an increase in the number of GCs compared with that of the control specimens (Figures 3(a) and ?and4).4). This increased number of GCs (Figure 3(c)) was accompanied by an enhanced production and release of mucus, such that the apical surface of these cells protruded above their neighboring ciliated cells, with their contents liberated into the lumen (Figure 3(c)). No increase in the number or activity of GCs was observed when nasal septa were incubated separately with each lectin. Figure 3 Toluidine blue staining of respiratory nasal epithelia of rabbit fetuses exposed to P. multocida. (a) Normal epithelium not exposed to bacteria or lectins; LM, scale bar = 20?��m. (b-c) Nasal septa of positive control tissues exposed only ... Figure 4 Inhibition of the increase in the percentage of GCs in the respiratory epithelia of nasal septa treated with various lectins. *Significant inhibition (P