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zealandica: LLT: ?5, ?6, ?7, ?13, ?14, ?15 ��C, ULT: 35, 37, 39 ��C; S. yirgalemense: LLT: ?5, ?6 ��C, ULT: 35, 37, 39, 41, 43 ��C). We also added the control replicate data from these acclimation experiments back to our ULT and LLT datasets to increase their sample size. Statistical analysis All statistical analyses were performed in R (version 3.1.2; R Core Team, 2014). For the upper and lower lethal temperature experiments, without acclimation treatments, we modelled percentage survival as a function of observed temperature for both species separately using generalised linear models (glmfunction) with a binomial distribution and a logit link function. We tested for over-dispersion using the dispmod (version 1.1) package and then rescaled deviance to 1 when necessary. We then calculated LT10, LT50 and LT90 SWAP70 temperature profiles for each species using the dose.p function in the MASS package. For the acclimation experiments, we again considered each species separately and performed generalised linear models using binomial distributions and logit link function. In this case we used set temperature rather than observed temperature so that we could examine acclimation between cohorts, and then added acclimation treatment as an additional effect and an interaction term between acclimation and set temperature. Temperature and acclimation treatments were used as categorical variables and then Wald��s ��2 test in the ��arm�� package was used to test the significance of these predictors. Results Heterorhabditis zealandica and Selleck NVP-BKM120 S. yirgalemense displayed contrasting lethal temperature responses (Fig. 1). Overall, S. yirgalemense displayed greater survival at higher temperatures than H. zealandica (simplified Wald z = ? 4.48, p selleck Linear Model summary for the effect of temperature on upper (ULT) and lower lethal temperature (LLT) limits of Heterorhabditis zealandica and Steinernema yirgalemense. Table 2 Generalised Linear Model/logit model predictions for 10, 50 and 90% survival of the population (i.e., LT90, LT50, LT10) of Heterorhabditis zealandica and Steinernema yirgalemense at upper (ULT) and lower (LLT) temperatures. For both species, acclimation of thermal limits in both species resulted in both negative and positive responses to experimental temperatures as seen in both lower and higher survival limits at different temperatures (Fig.