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Figure 1 Total Phenolic (mg GAE/100 g) Figure 2 Ferric reducing antioxidant properties Figure 3 1, 1- Diphenyl-2-picrylhydrazyl (%) Figure 4 (%) Hydroxyl radical The ability of the extracts to chelate Fe2+ when incubatedwith 150 mM FeSO4 is shown in Figure 5. Ethylacetate showed the highest percentage chelating ability with 60.23 �� 0.07%; with ethanolic extract showing 36.24 �� 1.62%, and the least which is the aqueous extract with 14.49 �� 0.01%. The extracts when incubated with 5% NaNO3 to determine the flavonoid content as shown in the Figure 4. It is clearly shown that ethylacetate extract with 1.146 �� 0.01 has the highest percentage flavonoid content with ethanolic and aqueous extracts showing almost equal flavonoid values with 1.044 �� 0.01 and 1.037 �� 0.01%, respectively [Figure 6]. Figure 5 Fe chelation (%) Figure 6 Total flavonoid (%) Figures ?Figures77�C9 relates the antioxidant enzymes parameters learn more that were carried out with 100 mg/kg of the aqueous extract of T. triangulare to determine the effect (s) of the extract at this relatively minimum dosage on the antioxidant enzyme activities in the hepatic, renal, and the cerebral tissues. Antioxidants carry out their protective roles on cells in one of the ways either by preventing the production of free radicals or by neutralizing/scavenging free radicals produced in the cells or by reducing/chelating the transition metal composition of vegetable.[6,23] Aerobic cells are made up of extensive cellular antioxidant defense mechanisms which Laccase includes both low molecular weight scavengers which prevent the damaging effects of toxic oxygen species,[24] and high molecular weight endogenous antioxidant enzyme such as SOD which converts the superoxide free radical anion to hydrogen peroxide and which is the most effective when its activity is followed SLx-2119 solubility dmso by that of CAT and GPx. CAT is capable of scavenging the hydrogen peroxide radical that is formed during various metabolic reactions in the cells. The GSH is involved in many important cellular functions, ranging from the control of physiochemical properties of cellular proteins and peptides to the detoxification of free radicals.[25] However, when there occurs imbalance between these parameters and ROS, a state of oxidative stress is occasioned, possibly leading to establishment of cellular impairment. From Figures ?Figures77-?-99 below, the values of the enzyme activities were analyzed and from the table the correlation among GSH, GPx, SOD, and CAT activities on the tissues were relayed and clearly shown from the figures how the extract has proven to cause increase in GPx in the organs (i.e. the kidney, liver, and brain) compared to that of the normal group. Control and the tests results observed for GPx in the brain were 4.46 �� 3.88