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Snap-frozen mouse brain specimens were obtained, and frozen coronal brain sections (20 ��m) were prepared on slides with a cryostat at ?20 ��C. The slides were warmed to room temperature and briefly air-dried, and were incubated with the buffer (0.01?mol/l Na2HPO4, 0.15?mol/l NaCl, pH 7.4, 0.1% bovine serum albumin) containing one of the following solutions: (i) 0.16 ?mol/l 32P-FB4-pRNA; (ii) 0.16 ?mol/l 32P-scramble FB4-pRNA. After 2 hours GW3965 ic50 of incubation at room temperature, the slides were washed with the buffer and air-dried, followed by exposed to phosphor screen in a cassette overnight and then scanned by phosphorimager. Statistical analysis. Statistical analysis was performed by analysis of variance, followed by posttests (Dunett's test for comparison of experimental groups versus control conditions). P Target Selective Inhibitor Library nmr reanalysis of the data of the Women's Health Initiative (WHI) study along with the results of recent studies, have clarified the risks and benefits of systemic MHT. Current data indicates that MHT is beneficial when initiated in younger women closer to the onset of menopause, and in those women who take it for less than 10 years. The recommendations for MHT use have been provided by all Adenine the major professional organizations which include the International Menopause Society (IMS), the North American Menopause Society (NAMS), the European Menopause Society (EMAS), the British Menopause Society (BMS), and the Indian Menopause Society (IMS, India). It is important to keep in mind that MHT is a tool that affects the care of menopausal women not only during their transition years, but may extend over a longer period. During this phase, a woman faces many comorbid conditions which require consultation and treatment from multispecialty domains. As gynecologists, we look after the reproductive and sexual health of women during various stages of their lives. But as women develop many other comorbidities or chronic illnesses, we are faced with challenges in their management, especially with the use of MHT. In order to discuss few of these challenges, a Cross-Specialty Round Table Meeting with representatives from various disciplines related to women's health such as gynecologists, physicians, endocrinologists, rheumatologists, urologists, and oncologists was conducted by the IMS in 2014.