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The age of the logistic regression analysis, factors identified as independent predictors of clustering were for the first period this website homelessness (OR, 2.3; 95% CI, 1.2 to 4.5; p?0.011) and to be born in Spain (OR, 1.8; 95% CI, 1.2 to 2.6; p?0.002), and for the second only to be born in Spain (OR, 2.1; 95% CI, 1.4 to 3.0; p? >2?years the percentage of clustering decreased from 25% (15/60) in the first period to 23.5% (28/119) in the second (p?0.97). Analysis of the TB patients in mixed clusters Alectinib chemical structure indicated that in the first period 115 patients (77 Spanish-born and 38 foreign-born) were grouped in 21 clusters, and the index case was a foreign-born patient in 10 (47.6%). We estimated that 5.6% (22/396) of Spanish-born cases were attributed to recent transmission from foreign-born patients. In the second period, 72 patients (46 Spanish-born and 26 foreign-born) were grouped in 19 mixed clusters, and the index case was a foreign-born patient in 10 (52.6%). We estimated that 4.5% (16/352) of Spanish-born cases were attributed to recent transmission from foreign-born patients. In the period 2002�C2004 three mixed clusters accounted for 21.1% of clustered patients and in the period 2005�C2007 the three bigger mixed clusters accounted for 13.1% of clustered patients. Tryptophan synthase The percentage of cases included in multinational clusters (three or more nationalities) also decreased (5.6% vs. 2.3%; p?0.004). Epidemiological connections were established in the first period for 50 (22.0%) of the 227 clustered TB cases and in the second period for 39 (19.7%) of the 198 clustered TB cases (p?0.64). In both periods epidemiological links were established more frequently in clusters containing only foreign-born persons than in others clusters: 43.5% vs. 19.5% in the first period (p?0.02) and 38.5% vs. 15.1% in the second (p?0.002). Our study shows important differential findings when we analysed two consecutive 3-year periods in the epidemiological scenario of sustained increase of immigration.