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The immediate (atopic or anaphylactic) type of allergy is a function of serum antibodies belonging to the IgE class of immunoglobulins (22, 23). Specific IgE antibodies were measured by the ImmunoCAP specific IgE test (Phadia AB, Uppsala, Sweden), a well-established in vitro quantitative measurement used for the determination of IgE in serum or plasma; however, the test focus on the detection of inhalant allergens. According to Vidal et?al. (24), the test has a sensitivity of 70.8% (95% CI 61.7�C78.6%) and a specificity of 90.7% (95% CI 87.0�C93.5%) tested in an unselected adult sample, and tested in symptomatic patients 79.2% (95% CI 68.2�C87.3%) and a AZD6738 mw specificity of 91.6% (95% CI 85.6�C95.4%) and allows detection of very low concentrations of IgE antibodies (23, 25, 26). It allows quantitative measurements [in kilo antibody units per litre (kUA/l)] of specific antibodies against a mixture of the eight most common respiratory allergens, including those with the international allergen codes: g6-timothy grass, g12-cultivated rye, t3-common silver birch, w6-mugwort, d1-Dermatophagoides pteronyssinus, e1-cats epithelium and dander, e5-dog dander, m2-Cladosporium herbarum. Using the UniCap 100 apparatus (Phadia AB, Uppsala, Sweden), each standardized test needed 40?��l serum or plasma from the respective participant. The concentration of specific IgE was determined automatically according to the instructions of the ImmunoCap? (Phadia AB, Uppsala, Sweden) using ��0.35?kUA/l as the cut-off for a positive value. Finally, because Transmembrane Transporters modulator of missing of blood samples CASK for testing and invalid IgE measurements (n?=?46), specific IgE serum levels could be determined in 1462 blood samples. Thus, statistical analyses included 275 glioma cases and 528 controls, 175 meningioma cases and 343 controls, and 49 schwannoma cases and 92 controls. Odds ratios (OR) and 95% confidence intervals (CI) were calculated for all brain tumour subtypes, using conditional logistic regression models (27) (LOGISTIC procedure of the sas software package, Version 9.1; SAS Institute, Inc., Cary, NC, USA). Separate analyses have been performed for men and women because of considerable gender differences in brain tumour subtypes. Adjustments were made in all analyses for educational level (primary school or less, technical/secondary school, university degree and not specified or none educational degree) and for smoking status (never, former, current, unknown). Alcohol consumption (never, former, only at recruitment, lifetime, unknown), physical activity (inactive, moderately inactive, moderately active, active, unknown) and hormone replacement therapy (HRT) for women had only marginal influence on the risk estimates (