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If chosen arbitrarily, a given initial speed can cause loss of the LT1 data. Data from previous studies have shown that the initial speed chosen for incremental tests is sometimes very close to the speed corresponding to the LT1 (Castagna et al., 2013; Manzi et al., 2009). For instance, in the study of Castagna et al. (2013), the speed range at BLC of 2 mmol��L?1 was from 10.9 to 12 km��h?1, and the treadmill test started at a speed of 9 km��h?1. In the same way, in the study of Manzi et al. (2009), the mean speed at BLC of 2 mmol��L?1 was 11.1 (0.7) km��h?1 whilst the initial speed of the test was 10 km��h?1. These studies did not mention lost data due to the fact that the protocol started at a speed very close to the LT1 intensity, but considering a 95% confidence interval, it is likely that some athletes may have reached BLC ��2 mmol��L?1 in the first stage, as already mentioned in a previous study (Davis, Caiozzo see more et al., 1983). Thus, our study provides an objective criterion for choosing the intensity to start an incremental treadmill test, avoiding an overestimation of initial speed. An initial speed close to 70%AVSRace did not underestimate the ideal speed to start incremental tests when the reference was the speed in half marathons or 10 km races, but it could underestimate the ideal speed if the reference was the speed in marathons. However, Akt inhibitor the volunteer who had BLC exactly equal to 2 mmol��L?1 presented an initial speed lower than 70%AVSRace in relation to his highest race speed. Therefore, if the evaluators had chosen to start the test at an intensity corresponding to 70%AVSRace before the experiment, they would have ensured BLC 1 mmol��L?1 during the first stage, even though many of them performed at a speed Montelukast Sodium short, the probability of reaching BLC