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21 Body dimensions Anthropometric measurements included height (cm) and weight (kg) and were measured on all three test occasions. Body mass index (BMI) was calculated as body weight per squared body height (kg �� m?2). Upper body pain Information on prevalence of symptoms in the neck and shoulder region was obtained through answers to two questions from the standardized Nordic Musculoskeletal Questionnaire: ��Have you at any time during the last 7 days had trouble (ache, pain, or discomfort) in your neck?�� and ��Have you at any time during the last 7 days had trouble (ache, pain, or discomfort) in your shoulders?��.26,34 These two questions about neck and shoulder pain, respectively, were merged into upper body Itraconazole pain (UBP). Statistical methods Mean and SD for continuous and normally distributed variables, the median and range for nonnormal variables, and percentages for dichotomous variables were used to describe the measurements in the cohort. Sex differences were analyzed using the chi-square tests for dichotomized data and the Student��s t-test for independent-samples for continuous data. The analyses were performed in SPSS 21.0. All effects were considered significant with a P-value selleck chemicals llc tests were not normally distributed and were therefore transformed for the statistical analyses. The variable BMI was lg10 transformed, and the variable curl up was square root transformed to obtain normally distributed data. Univariate logistic regression analyses were used to test which of the independent variables were significantly associated with UBP (dependent variable, 1= having UBP at the age of 52 years). Sex was included as an interaction variable together with each of the other variables. Thereafter, multiple logistic regression analysis was performed to investigate which variables could predict UBP at the age of 52 years. Only variables that were significantly associated with UBP in the univariate analyses and that were measured at the ages of 16 and 34 years were included as independent variables in the analysis. All participants who had performed the tests and/or answered the questionnaires were included in the analyses. Vandetanib clinical trial Results Background characteristics Characteristics of the cohort participants who answered the questionnaire and performed the physical fitness tests in 2010 (n=213) are presented in Table 1. Approximately 20% of the cohort participants reported UBP. There were no significant differences between men and women regarding UBP, aerobic capacity, flexibility, balance, or work-related factors. Regarding muscular strength and endurance tests, men had significantly higher results in the two-hand lift and curl-up tests compared to women.