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This was very different from Study 1, in which participants came to a university campus, were seated in a quiet room facing the experimenter and assistants, and met other participants. As even subtle cues of the existence of another person, such as eye-like paintings, can influence behavior in experimental economic games (Haley and Fessler, 2005; Burnham et al., 2007; Oda et al., 2011; Nettle et al., 2013), the difference between the group experiment and the web experiment was enormous. Second, as participants recorded a conditional decision on the response page, the expected payoffs for the participants and other members were explicitly Cilengitide research buy indicated. Third, the game rule was changed so that the aggregate contribution was doubled and distributed equally among the four group members. In other words, the return rate of the investment was larger (0.5 times for Study 2 and 0.4 times for Study 1). Fourth, we asked the participants to register C0 decisions, which we failed to collect in Study see more 1. Fifth, there was no show-up fee for Study 2. The second and third changes were intended to make it easier for participants to understand the game structure. Registered responses were randomly grouped and game payoffs computed. The results were sent to the participants via postal mail. By the same mail, participants were asked to send back their bank account information, so that payoffs could be transferred to them (1 point = ?20). All the procedures were explained before participants logged in to the response webpage. In preparation for the experiment with twin participants, we conducted a preliminary experiment with undergraduates (n = 37; Hiraishi, unpublished data). The results were generally consistent with Fischbacher et al. (2001) study; we observed the two major strategies of conditional cooperation (n = 17) and free riding (n = 16). The experimental procedures were approved by the ethics committee at the Faculty of Letters, Keio University. Results Simple Statistics We found that as the contribution by others increased, both the mean contribution decisions and the variances of the conditional decisions increased (Table ?Table44). Regression of mean contribution decisions on others�� contribution showed significant positive relationship Non-specific serine/threonine protein kinase (b? = 0.996, p