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In addition, the area under the receiver operating characteristic curve (AUC ROC) was calculated as indices of the fit of the model. All the statistical analyses were conducted using R 2.10.1, and maps were produced with a Geographic Information System (ESRI1, ArcGISTM 9.0). Results The stables with at least one positive animal considered as positive stable and 108 of 260 stables (41.5%) were positive for WNV infection. In the training set, on Entinostat ic50 univariate analysis, all the variables were associated with the presence of WNV antibody (Pclick here respectively. The average minimum and maximum annual humidity were 25.6% and 83.8%, respectively. Annual mean humidity for infected places was 44.57% (95% CI 42.07�C47.06) and for uninfected places was 47.94% (95% CI 45.98�C49.89). The average minimum and maximum annual Mianserin HCl precipitation were 50.8 mm and 1759.1 mm, respectively. Annual mean precipitation for infected places was 258.74 mm (95% CI 210.58�C306.91) and for uninfected places was 299.18 mm (95% CI 266.83�C331.53). The risk of being WNV seropositive increased for stables located in the area with higher temperature, as 5 ��C increase in mean annual temperature was associated with a statistically significant 100% higher prevalence of WNV infection. Fig. 3 Fig. 3 shows the aggregation of infected stables within the area with higher temperature. Fig. 3. Annual mean temperature and geographical distribution of WNV infected and uninfected stables in Iran No significant association was observed between type of stable, annual mean humidity, precipitation, annual mean NDVI and seropositivity. It is notable that type of stable is different from their location, as the former more is indicating the management of the stable and the latter indicating geographic location. There was a significant negative correlation between distance to the nearest wetland and seropositivity of stables. The risk of being WNV seropositive for stables increased (27%) with 10km decrease of distance from the nearest wetland area (Fig. 4). Fig. 4.