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The inclusion criteria of the control group were as follows: LVEF ��50% and agreement by the patient to provide blood samples from the CS, aortic root and peripheral vein. The exclusion criteria for the control group were as follows: LVEF Sclareol of myocardial infarction or disease causing increased myocardial oxygen consumption (hyperthyroidism and anemia); and refusal of the patient to provide blood samples from the CS, aortic root and peripheral vein. The experimental group consisted of 31 males and 13 females. The control group consisted of 16 males and 18 females. The average ages of the individuals in the experimental and control group were 60.27��10.65 and 54.97��11.96 years, respectively. The experimental group included older patients and a greater proportion of male patients. Baseline information for the two groups is shown in Table I. Table I. Baseline characteristics. Method of extracting blood samples All the patients this website were prepared to undergo either coronary angiography or radiofrequency catheter ablation. Two sheathes were placed in the femoral artery and femoral vein and blood samples were extracted from the aortic root, CS and femoral vein using a coronary angiographic catheter. All the patients with HF were treated with anti-HF drugs and their cardiac function improved to a classification of NYHA II. Prior to extracting blood from the CS, the X-ray position was 30�� relative to the left anterior oblique position. Under fluoroscopy, the catheter reached the upper right atrium and turned toward the spine. Subsequently, the operator slowly pulled back the catheter. Jumping of the catheter signified that the catheter's tip had potentially reached the CS. The operator used a contrast agent during angiography to ensure that the catheter was within the CS. Blood gas analysis Following the withdrawal of blood samples from three different places, the samples were twice subjected to blood gas analysis. The result of each blood gas analysis is expressed as an average number. The machine used for blood gas analysis was the Abbott i-STAT EG7+ (Abbott, Abbott Park, IL, USA). Statistical analysis Statistical analyses were performed using SPSS 17.0 (SPSS, Inc., Chicago, IL, USA). Continuous variables were expressed as the mean �� standard deviation and analyzed using independent sample click here t-tests. Correlation analyses and regression analyses were also performed. P