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Transfusions of whole blood or packed red blood cells were administered whenever the PCV dropped below 12% and on the basis of clinical examination findings and an assessment of the rate at which the PCV had decreased over time. Cross-matching was not performed for animals that Everolimus purchase had not previously received a transfusion but this was carried out for all subsequent transfusions. A population of 84 control animals was assembled by selecting those animals with the case numbers on either side of the IMHA cases. The age, breed and sex of these animals were recorded. All statistical analyses were performed using SPSS (version 16, SPSS Inc, IBM Company) with significance designated as P��0��05, unless Cilengitide otherwise stated. Binary logistic regression was used to determine whether dogs with IMHA were more likely to be of a particular age, breed or sex. Variables (containing data from the control and test populations) were stratified into suitable groups and entered into separate models as categorical variables. For the purpose of the analysis, animals were grouped according to the treatment they received: group A received prednisolone (Prednidale; Dechra Veterinary Products) and cyclosporine (Atopica; Novartis Animal Health), group B received prednisolone and azathioprine (Azathioprine; Generics [UK] Ltd) and group C received only prednisolone. Survival data were analysed using a Kaplan-Meier procedure stratified according to treatment group. Survival time was measured from the date of admission to the hospital to the date of known death or euthanasia or, for censored data points, to the date on which follow-up data were obtained. The percentage mortality figures for the different treatment groups during hospitalisation, Palbociclib research buy at 1 month after discharge from the referral institute and at 1 year after discharge were compared using a chi-squared test. Where significant differences were detected, z tests were performed to compare proportions between treatment groups. Differences between treatment groups in terms of other variables were further assessed using Student��s t tests and Mann-Whitney U tests for parametric and non-parametric numerical data, respectively. Chi-squared tests were used for categorical variables and Shapiro-Wilks tests were used to classify variables as parametric or non-parametric. Comparisons of animals that survived or died at different time points and associations between corticosteroid dosage and prevalence of adverse effects were assessed using the same tests. To identify putative prognostic factors for the outcome of cases, data from variables collected on admission were evaluated using a Cox proportional hazard model. Individual variables (for which data from >90% cases were available) were first entered into a univariate model and retained if they were significant to the level P