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The -����Ct value was calculated by subtracting the -��Ct value of a normal sample from the respective -��Ct values of the patient samples. Expression of miR-1290 was normalized using the 2?����Ct method (13,14). The miR-1290 expression check details profile was used to create a receiver operating characteristic (ROC) curve, which is a graphical plot of the true-positive vs. the false-positive rate. The area under the ROC curve represents the discrimination accuracy. Statistical analysis Statistical analysis of the causal association between the clinical background and expression level of the miRNAs was performed using EZR software (15). A P-value of Fleroxacin miRCURY LNA microRNA array (Exiqon, Copenhagen, Denmark) in serum samples from 6 patients with cervical cancer and 6 healthy subjects to screen for candidate miRNAs associated with the development or progression of cervical cancer. The purpose of this analysis was to screen for a specific miRNA which may serve as a diagnostic or prognostic biomarker for cervical cancer patients. Initially, we compared miRNA expression in the serum of cervical cancer patients to that in the serum of healthy subjects. Of the 1,223 miRNAs compared in serum samples RO4929097 clinical trial from cervical cancer patients and healthy controls using a miRCURY LNA microRNA array, 6 were found to exhibit a >3.0-fold change in their expression level (P