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Data were integrated and scaled with XDS (Kabsch). The structure of the TmMRNBD complex was determined by SAD phasing methods using AUTOSHARP (Global Phasing, Cambridge) which located all 20 expected selenomethionine sites within the asymmetric unit. Prior to model building and refinement, we randomly omitted 5% of the reflections for monitoring the free R value. The model was manually built in the electron density using COOT ( Emsley MK-4827 and Cowtan, 2004). After corrections for bulk solvent and overall B values, data were refined by iterative cycles of positional refinement with CNS ( Brunger et?al., 1998) and TLS refinement with PHENIX ( Adams et?al., 2002), followed by manual model building with COOT. Data collection and model statistics are given in Table S1. All figures were prepared with PyMOL (DeLano Scientific). Crystals of TmMre11HLH(aa 343�C385):Rad50NBD were grown by the sitting drop vapor diffusion method and appeared after 9?days at 19��C. Data were integrated and scaled with XDS (Kabsch). The structure of the Mre11HLH:Rad50NBD:AMPPNP complex was determined by molecular replacement with Phaser (McCoy et?al., 2007) using several fragments of TmRad50NBD (see TmMRNBD complex above) as search models. Prior to model building and refinement, we randomly omitted 5% of the reflections for monitoring the free R value. The initial model was automatically rebuilt using ARP/wARP ( Langer et?al., 2008) and Buccaneer ( Cowtan, 2006) and manually completed with COOT ( Emsley and Cowtan, 2004). After CPI-1205 mw corrections for bulk solvent and overall B values, the model was refined by iterative cycles of positional and B factor refinement with PHENIX ( Adams et?al., 2002) and manual model building with COOT. Data collection and model statistics are given in Table S2. All figures were prepared with PyMOL (DeLano Scientific). For data processing and evaluation we used various programs of the ATSAS software package (Konarev et?al., 2006). We processed initial data with PRIMUS and calculated the radius of gyration (RG) using the Guinier approximation and the low-resolution (sxRG?DEF6 CRYSOL was utilized to compute theoretical SAXS-curves from crystallographic coordinate files and for comparison with the experimental data. Ab initio structure modeling was based on the DAMMIN reciprocal space fitting algorithm, assuming two-fold particle symmetry. Multiple models were generated and 10 models were aligned and averaged using SUPCOMB and DAMAVER. The final model was obtained by imposing a two-fold symmetry restriction based on the assumption that the crystal structure of the TmMRNBD heterotetramer comprises a twofold symmetry. Superposition of X-ray structures and bead models was carried out with DAMAVER.