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4,25 To determine the biochemical serum parameter of the both dependent and witness, case group, control and experimental group the following procedurals are performed. All groups of study were fasted for at least 12 h. To get the blood serum, we need about 5 mg of the newly gathered blood vein. Separating the serum 30 min after sampling and taking blood by centrifugation was in this case that the sample centrifuged for about 5 min/800 g. This blood serum will be frizzed in 20�� C and protected till the day of the experiment. The rate of the Na and K measured, through using electrolyte analyzer. Calcium (Ca) density measured through cresolphthalein complex method (Darman Kav Co. kit). Uric acid (UA) measured through the phosphotungstic acid method (Zist-Shimi Co. kit). And also, creatinine with the help of ��Jaffe�� method (Pars Azmun Co. kit), fasting blood sugar MRIP (FBS) density, through photometric and enzymatic (GPO-PAP) (Iran Shimi Co. kit) were evaluated. Cholesterol, TG, and blood urea nitrogen (BUN) were measured through photometric method (GPO-PAP) (Iran Shimi Co. kit). Colorimetric ��End point�� method (Zist-Shimi Co. kit) utilized for evaluation of the fibrino-tion as well as time needed selleck for thrombin were measure (coagulametric methods) (kit of the Estago Co. of France).4,25 The data were analyzed by SPSS for Windows 11.5 (version 11.5, SPSS Inc., Chicago, IL, USA) software. The group��s comparison performed through one-way ANOVA test. Toki-Kramer post-test was utilized for founding a meaningful place in between the above groups. The meaningful level of 0.05 was considered in this test. PD173074 mouse Moreover, data were delivered in the form of average of the �� standard deviation. Results FBS parameter indicated a meaningful decrease (P