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2009). Higher DH observed for hydrolysates produced by alcalase compared to trypsin was also reported by Ovissipour et?al. (2009) who compared the effect of four different enzymes (alcalase, trypsin, protamax, and neutrase) in the hydrolysis of Persian sturgeon viscera. The same results were also reported about fish peptone produced from tuna head (Safari et?al. 2012). However, the application of different hydrolysis time caused the production of protein hydrolysates with approximately equal DH for both enzymes. Table 2 Chemical characteristics of silver carp by-product peptones The soluble protein of the peptone obtained by alcalase was higher than the peptone produced by trypsin. The results were in agreement with results reported by others about alcalase efficiency in fish CP-673451 chemical structure by-product hydrolysis (Ovissipour et?al. 2009). However, Reissbrodt et?al. (1995) mentioned that to evaluate peptone efficiency in growth media, physical oxyclozanide and chemical data alone are not sufficient to understand the overall effect on microbial growth. Regarding the fact that peptones in microbial media provides the main sources of nitrogen and carbon, a successful peptone source is better evaluated by the growth of the organism of interest (Vieira et?al. 2005). Microbial growth curve The growth curves of S. aureus on three different media are shown in Figure?Figure1.1. As can be seen, the bacteria Decitabine research buy grew properly in all three media. However, the best growth rate was observed for the bacteria in the medium containing alcalase peptone compared to TSB and trypsine medium which showed significantly higher OD during the study period (P?