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All participants provided written informed consent. Inclusion criteria were as follows: participation in the ROOTS study; normal or corrected-to-normal vision; Northern European descent; English speaking. Exclusion criteria were: any history of neurological trauma resulting in loss of consciousness; current psychotropic medication use; current neurological disorder; current DSM-IV Axis selleck screening library 1 disorder; presence of metal in body; specific learning disability, and IQ?LMTK2 (PH) as diagnosed using K-SADS. Inline Supplementary Table S1. This semi-structured interview is conducted with the child's primary caregiver and records family-focussed adverse life experiences, child's age at occurrence, duration, and an interviewer assessment of their practical impact on the daily life of the family (see (Dunn et al., 2011) for more information). The current investigation Romidepsin cost used contextual data from the first eleven years of life to classify adolescents into those exposed (CA+) and not exposed (CA?) to early family adversities. Exposure to an adverse family environment was defined as exposure to abuse (emotional, physical or sexual) and/or significant family discord; occasional physical violence, lack of affectionate warmth, or severe lack of communication between family members. At ages 14 and 17, participants in the ROOTS cohort had completed a self-report measure of negative life events and difficulties (modified from (Goodyer et al., 2000)), occurring to them, their family or closest friends over the preceding 12?months. Participants were asked to date these experiences and rate their impact on themselves on a scale from 1 = very pleasant/happy to 5 = very unpleasant/sad/painful. If participants rated either 4 or 5 they were asked to indicate if they felt upset for longer than 2?weeks. From these ratings, two separate summed totals for positive and negative recent life events rated as occurring for longer than 2?weeks were derived. The negative event ratings were used here. We have shown acceptable test-retest reliability for the reporting of recent negative events (��?=?0.75), significant agreement (��?=?0.8) between maternal and adolescent perceptions of occurrence (with the exception of sexual behaviours, which are usually not reported by parents), and high agreement (��?=?0.