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24 Suitability of the methods towards the estimation of bulk drug checked and found the mean recovery of 98.88?��?0.45% this high percentage recovery proved that the method can adoptable for the estimation of TL in bulk. For the application of the proposed method to formulation the procured tablets were subjected to the analysis for their contents of TL by the selleck chemicals proposed method and reported UV spectrophotometric method reported by Nanda et?al.7 From test conducted about 99.91 and 99.67% assay was resulted with the proposed and existed method (Table 2). The results obtained are given in Table 3. The percentage relative standard deviation (% RSD) for inter, intra-day precision was about 0.898 and 0.945 respectively which was very low and within the acceptance limits for precision experiments, evidencing repeatability (precision) of the method. The resulted recovery at three levels was with the % RSD of 0.94�C0.98% for TL (Table 3). The above % RSD were found within the acceptance limit for accuracy of HIF inhibitor studied excipients do not cause any interference in the estimation of the drug (Table 4). Likewise the placebo mixture of above excipients was prepared without the drug and studied at the wavelength of estimation for determining any absorbance for the chloroform extractable material in the placebo. Yellow color was not developed in?the extract revealed the selectivity of the present method. Likewise the results of stability form the shown from Fig. 7 evidenced that the chromogen oxyclozanide was stable more than 3.5?h. The results obtained were within the suggested limits for % RSD (