An 2-Minute Strategy For SERCA

05) and a substantial reduction in the duration of expiration (322 �� 31, versus saline, 483 �� 23 ms; P 0.05; Fig. 2C and D�CE). The injections of muscimol (2 mm in 50 nl) were placed bilaterally into the RTN/pFRG of eight unrestrained, awake rats (Fig. 3A). Their rostrocaudal scatter is shown in Fig. 3B. SERCA The inhibition of the RTN/pFRG bilaterally with muscimol reduced the tidal volume (1.1 �� 0.3, Luminespib cell line versus saline, 2.3 �� 0.2 ml kg?1; P 0.05; Table 2). The following experiments were designed to determine whether bilateral inhibition of the RTN/pFRG might suppress the effects of central chemoreceptor activation on arterial pressure, tidal volume, breathing rate and minute ventilation. Unrestrained awake rats (n= 8) were exposed for 10 min to hypercapnia (8�C10% CO2 in the inspired air). Bilateral injections of muscimol click here (2 mm in 50 nl) into the RTN/pFRG reduced the increase in tidal volume (3.2 �� 0.5, versus saline, 7.8 �� 0.3 ml kg?1; P 0.05) and HR (��=?4 �� 3, versus normocapnia, ��=?6 �� 2 beats min?1; P > 0.05; Fig. 3C and Table 2). Complete recovery from the cardiorespiratory effects of muscimol occurred within 120 min (Table 2). The effects of muscimol injected into the RTN/pFRG in the responses to hypoxia (8�C10% O2 in the inspired air for 10 min) during normocapnia were tested in the same animals used to test the effects of hypercapnia (n= 8). Bilateral injections of muscimol (2 mm in 50 nl) within the RTN/pFRG reduced the increase in tidal volume (4.7 �� 0.4, versus saline, 8 �� 0.3 ml kg?1; P