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The images of motor endplates collected from the sections were uploaded to ImageJ (Version 1.45s) and the length of each motor endplate was measured. Motor end plates lengths are presented as mean?��?SEM. Statistical analyses were performed using GraphPad Prism learn more (version 3.02, GraphPad Software, Inc., La Jolla, CA). MTS assay, neurite assessment and PGP9.5 immunolabeling were analyzed by one-way ANOVA with Tukey's multiple comparison test. The toe spread test were analyzed with repeated measures ANOVA with time as a within-subjects factor, groups (LT and control) as a between-subjects factor, and ratio to baseline as the dependent variable. Dunnett's multiple comparisons test was used to compare each time point to week 4 separately for each group, and Sidak's multiple comparisons test was used to compare groups at each time point. Familywise error rate was set at alpha?=?0.05. The motor end plate data was analyzed by one-way ANOVA with Tukey's multiple comparison test. A statistically significant difference was defined as P value less than 0.05. The effects of altering time of irradiation at a power density of 10?mW/cm2 for two different wavelengths of light on in vitro human fibroblast mitochondrial metabolism are summarized in Figure 1. The laser had an aiming beam that remained on during laser treatment. Entinostat order Therefore, an experimental group was included (aiming beam group) which was irradiated with the aiming beam only. These cells were treated the same as the 980?nm wavelength group. There was no statistical difference in the mitochondrial metabolism of the non-treated controls and the fibroblasts treated with the aiming beam as measured by mitochondrial dehydrogenase activity using the MTS assay. For the range of energy densities investigated, 810?nm wavelength light at an energy density of 5,000?mJ/cm2 caused a statistically significant increase in mitochondrial metabolism. Over the same range of energy densities, 980?nm Mianserin HCl wavelength light caused a statistically significant decrease in mitochondrial dehydrogenase activity for all energy densities tested. This finding is important and illustrates that it cannot be assumed that laser parameters optimized for one wavelength can be used for other wavelengths. To determine effective energy densities for 980?nm wavelength light, a series of experiments was done using in vitro rat cortical neurons. The neurons were subjected to high glucose concentrations to cause impairment of their metabolism and neurite extension. The glucose concentration used in these experiments was established in a preliminary study in which it was found that a 28% decrease of total neurite length (P?