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We applied Scanning Natural Product Library Electron Microscope to evaluate the morphological features of endothelial cells on titanium substrates. The mRNA expression of NGF and its receptors (TrkA, p75) and angiogenic related genes (VEGF) were measured by real-time PCR. For in vivo assay, each groups�� cell culture supernatants were added to Chicken embryo chorioallantoic membrane (CAM) of 8th day fertilized eggs and cultured for another 24?h. Then the CAMs were fixed and transfer each specimen to petri dishes. The images were acquired and analyzed with Image Pro Plus. Results NGF could induce ECs proliferation on titanium substrates from day 3 to 7. For the morphological features, ECs are ball-like on titanium surface at 2?h. While at 24?h, the cells were totally stretched and fused into the rough titanium backgrounds. Nerve growth factor could up regulate the mRNA expression levels of NGF, TrkA, p75, VEGF. In vivo, the supernatants of NGF group could induce more neovascularization in CAM membrane. Conclusion and clinical implications This study first time demonstrated that NGF could enhance ECs proliferation, genes expression on titanium surface and more neovascularization in CAM. This may provide Aldosterone a novel thought for promoting early dental implant osseointegration in clinical. Further experiments and clinical trials to elucidate mechanism of Nerve growth factor on angiogenesis and osseointegration of peri-implant are needed. ""To measure in vivo impact of dense bone overheating on implant osseointegration and peri-implant bone resorption comparing different bur irrigation GDC-0973 price methods vs. no irrigation. Twenty TI-bone? implants were inserted in the inferior edge of mandibles of sheep. Different cooling procedures were used in each group: no irrigation (group A), only internal bur irrigation (group B), both internal and external irrigation (group C), and external irrigation (group D). The histomorphometric parameters calculated for each implant were as follows: %cortical bone�Cimplant contact (%CBIC) and %cortical bone volume (%CBV). Friedman's test was applied to test the statistical differences. In group A, we found a huge resorption of cortical bone with %CBIC and %CBV values extremely low. Groups B and C showed mean %CBIC and %BV values higher than other groups The mean %CBV value was significantly different when comparing group B and group C vs. group A (P?