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The microballoons were adhered to an aluminum stub using a double adhesive tape. The stubs were then coated with silver under an argon atmosphere using a high-vacuum evaporator (Polaron SEM coating system). The photomicrographs of coated samples were taken randomly. The release rate from different fractions of the GSK J4 optimized formulation was determined using USP type II apparatus. Dissolution medium (SGF, pH 1.2, 500?ml) containing 0.02% Tween 20 was filled in the dissolution vessel and stirred at 50?rpm. The temperature was maintained at 37?��?0.5?��C. A weight of microballoons equivalent to 50?mg of amoxicillin was placed in the dissolution vessel. Aliquots were withdrawn at every 15?min of the first hour and then at every hour till 4?h followed by 6 and 82?h. Samples were then analyzed by UV-spectrophotometer (UV 3000+, LabIndia Instruments, Mumbai, India) at 228?nm. All the dissolution runs were conducted in triplicate. The release behavior of all the three fractions was compared with the optimized formulation (reference formulation) [13]. The mechanism of drug release from the microballoons was confirmed Proteases inhibitor by Peppas' equation. Differences in in?vitro drug release of amoxicillin from different fractions of the optimized formulation were statistically analyzed by one-way analysis of variance (ANOVA) with posttest (Tukey's multiple comparison test). Statistically significant difference between in?vitro amoxicillin release from different fractions was defined as p?S6 Kinase to tubes containing 1?ml of Mueller-Hinton broth at 5, 10, 20, 30, 40 and 50?��g/ml concentrations. Tubes were inoculated with 10?��l H. pylori suspension prepared in saline and incubated in the microaerophilic environment (CO2 10%, O2 5%, N2 85%) at 37?��C for 5 days. The inoculum was prepared to contain 108?CFU/ml by adjusting the suspension to match the McFarland No 0.5 turbidity standard. The growth was observed as turbidity in the samples (+) and inhibition of growth was confirmed by the transparent appearance of medium (�C). The duration of growth inhibition was also determined for a period of 84?h. Controlled release system of amoxicillin was designed and optimized to increase its residence time in the stomach without contact with gastric mucosa for effective eradication of H. pylori infection. Novel hollow microballoons were prepared via emulsion solvent diffusion method.