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The samples were coded for each participant and testing session. Relaxin (hRLX; Immundiagnostik AG, Bensheim, Germany; sensitivity MLN8237 performed and?P-values Bonferroni corrected to examine any differences highlighted. Spearman correlation coefficients were determined to examine the relationship between serum relaxin levels and tendon mechanical properties. Significance was set to?P?C59 for PTL, 0.98 for MGTL and 0.98 for relaxin level. There were no significant differences in the level of serum relaxin, the cross-sectional area of the tendons, or the mechanical properties of either the medial gastrocnemius or patellar tendons across the three phases of the menstrual cycle (P?> 0.05; see Tables 1 and 2 for values). Aldosterone Relaxin concentrations were seen to account for between 26 and 31% of the variation in patellar tendon stiffness. Significant correlations were found between serum relaxin levels and stiffness, for the patellar tendon only (r?=?0.561,?P? 0.05) of the variance in tendon stiffness in the medial gastrocnemius, but these associations were not significant. There were no significant associations between tendon cross-sectional area and relaxin levels (r?=?0.193,?P?> 0.05 in the patellar and?r?=?0.233,?P?> 0.05 in the gastrocnemius tendons). The present study shows that in a group of normally menstruating women (not taking any form of medical contraception), the menstrual cycle impacts neither on relaxin nor on the structural and mechanical properties of the medial gastrocnemius and patella tendons (P?> 0.05). However, a significant relationship (P?