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0010; Pcorrected?=?0.015; OR, 0.81; 95% CI, 0.71�C0.92) (Table?2). Another four SNPs, rs9303277 [9, 17, 19], rs7224129 [19], rs3744246 [17], and rs4794820 [9, 19], associated with AR have been reported as susceptibility SNPs for asthma at genome-wide significant levels (P?selleck chemicals we combined the two studies and observed significant associations between the five SNPs and AR (Pcorrected?=?0.012�C0.044). Haplotype frequencies for Y-27632 molecular weight associated variants were estimated, and five common haplotypes covered more than 98% of the population in the both case and control groups. We could not find a specifically significant haplotype association with AR (P?=?0.90�C0.00050). The most significant association was observed with haplotype T-C-G-T-A (rs9303277, rs7216389, rs7224129, rs3744246, and rs4794820) vs others (P?=?0.00050). We further investigated correlations between serum total IgE levels and genetic variations within patients with AR by the Jonckheere�CTerpstra trend test. However, we could not find any correlation (P?=?0.39�C0.98) between the 15 tag SNPs and serum IgE levels of patients with AR in this study. It has been reported that the associated variants of the 17q21 locus influence the expression of the ORMDL3 gene in Epstein�CBarr virus (EBV)-transformed lymphoblastoid cell lines from children in an asthma family panel [9]. Therefore, we evaluated whether the Fleroxacin related variants could affect the mRNA expression level in EBV-transformed lymphoblastoid cells. After isolation of total RNA from 69 cell lines, the expression of genes in the 17q21 asthma susceptibility locus including the ORMDL3, GSDML, ZPBP2, and IKZF3 genes was determined by real-time quantitative PCR using SYBR Premix Ex Taq II. Asthma-associated polymorphisms at 17q21 influence cord blood ORMDL3 and GSDMA gene expression [24]; however, we did not examine GSDMA because its expression level is very low in lymphoblastoid cells (data not shown). Although the GSDML, ZPBP2, and IKZF3 transcripts were not correlated with genotypes of associated SNPs (data not shown), the expression level of the ORMDL3 transcript was significantly correlated with the rs7216389 genotype (P?=?0.0058) (Fig.?3). We also observed correlations between the ORMDL3 expression levels and other genotypes of associated SNPs rs3744246, rs4794820 (P?