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Mice received five consecutive trials per session, one session per day (?30?s between NLG919 datasheet trials). Open field chambers were 40?�� 40?cm (Med Associates, St. Albans, VT, USA) with lighting at 21 lux. Infrared beams recorded the animals�� locomotor activity and rearing movements (vertical activity). Data were collected in 5?min bins during 45?min sessions for 3?days. For the treadmill performance, a plexiglass enclosure was placed over the treadmill to force the mice to remain on the track during the trials (enclosed treadmill space, 5?�� 20?cm). Mice were provided three 20?s trials per day for 3?days at incrementing speeds (10, 15, and 20; 15, 15, and 20; 15, 20, and 20?cm/s, days 1�C3, respectively). Their performance was assessed on the final day at 15 and 20?cm/s by recording the amount of time the animals remained in the forward two-thirds of the track versus Tubulin falling back into the back?third. All injections were intraperitoneal (i.p.) at 0.01?ml/g of body weight. SCH23390, eticlopride, and theophylline (Sigma-Aldrich, St. Louis, MO, USA) were administered 30?min prior to sessions at the specified doses prepared in 0.9% saline. After isoflurane anesthesia, animals were decapitated, and their brains removed to ice-cold sucrose aCSF (in mM: sucrose 125, KCl 2.5, MgCl2 1, CaCl2 2.5, glucose 20, NaH2PO4 1, NaHCO3 25, ascorbic acid 10; bubbled with 95% O2/5% CO2). Coronal slices were cut (250?��M; VT1000S, Leica) containing the dorsolateral striatum (+0.4?mm-+1.0?mm from bregma) and removed to a holding chamber perfused with normal aCSF (in mM: NaCl 125, KCl 2.5, MgCl2 1, CaCl2 2.5, glucose 20, NaH2PO4 1, NaHCO3 25, ascorbic acid 1; bubbled with 95% O2/5% Verteporfin mw CO2), 20?ml/min at 34��C. For recording, the slices were superfused with normal aCSF without ascorbic acid at 2?ml/min, 32��C. In the dorsolateral striatum D2-GFP MSN��s were visualized using fluorescence illumination on an upright microscope (Axioskop, Zeiss, Oberkochen, Germany). Whole-cell voltage clamp recordings used an Axopatch 200B amplifier, a Digidata 1200 interface, and pCLAMP 8 (Molecular Devices, Sunnyvale, CA, USA). All recordings were filtered at 1 kHz and digitized at 5kHz, Vm?= ?70?mV. For all recordings we used borosilicate electrodes (3�C7 M��) containing (in mM), K-gluconate 154, KCl 1, EGTA 1, HEPES 10, glucose 10, and ATP 5 (pH 7.4 with KOH). Only cells with series resistance