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Labelling DC with SPIO reflects the kinetics of DC migration in vivo but appears to reduce overall DC migration, in part due to nanoparticle size. Magnetic separation of SPIO-labelled (SPIO+) DC from unlabelled (SPIO?) DC prior to injection improves SPIO+ DC migration to the lymph node. Corresponding MR image data better correlate with the presence of DC in vivo; an improved immunological response is also seen. Cellular MRI is a viable, non-invasive imaging tool that can routinely track DC migration in vivo. Consideration should be given to optimizing MRI contrast agent-labelling of clinical-grade DC in order to accurately correlate DC fate to immunological outcomes in patients. Copyright ? Venetoclax 2011 John Wiley & Sons, Ltd. ""63162" "Maghemite particles are used as T2 contrast agents for magnetic resonance imaging, especially for molecular and cellular imaging. Linear clusters of particles �C called nanoworms �C were recently developed to enhance the targeting efficiency. In this work, the magnetic and NMR relaxation properties of these nanoworms are studied at multiple magnetic fields. After the usual saturation at 0.5 T, the magnetization of the worms is still increasing, which results in an appreciable increase of the transverse relaxivity at high magnetic fields. The obtained relaxivities are typical of superparamagnetic particles of iron oxide click here (SPIOs). The transverse relaxation of the worms is clearly more efficient than for the isolated grains, which is confirmed by computer simulations. At high field, the longitudinal relaxation of the worms is less pronounced than for the grains, as expected for SPIOs. The nanoworms thus constitute a promising T2 agent for cellular and molecular imaging. Copyright ? 2010 John Wiley & Sons, Ltd. ""63163" "This study was undertaken to investigate the effect of a magnetic resonance imaging (MRI) contrast agent, superparamagnetic iron oxide nanoparticle (SPIO), on signal transduction by glucose and insulin in pancreatic ��-cells. INS-1 cells were labeled in culture medium containing clinically approved SPIO for 24?h. Labeled and unlabeled cells were stimulated with glucose (25?mM) or insulin (0.1�C1??M) for 12?h. The phosphorylation of extracellular signal-regulated kinase1/2 (ERK1/2) and protein kinase B (AKT) Histone demethylase and intracellular insulin protein levels were assessed by Western blotting. After labeling with increasing amounts of SPIO, cytotoxicity was not observed, yet the intracellular iron concentration increased in a dose-dependent manner. SPIO labeling (200??g Fe ml?1) induced a significant increase in ERK1/2 and AKT phosphorylation (labeled vs unlabeled, p?