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Excluding the contribution of calcium from breast milk, however, would result in an overestimate for the prevalence of inadequate calcium intakes, but would not pose any risk for excessive Oxygenase intakes after fortification because the UL for calcium is so high (i.e. 2.5?g) (IOM 1997; WHO/FAO 2006). In addition, we recognise that our prevalence estimates for inadequate intakes of calcium, zinc and iron are all limited by uncertainties in the nutrient intake data and in the EARs set for infants and toddlers, as well as by our use of the EAR cut-point method to generate the estimates for calcium and zinc. For example, most of the dietary data were collected from the caregivers by 24-h recalls, although the portion sizes recalled as consumed were weighed to enhance their accuracy, and the nutrient values for iron, zinc, calcium and phytate content of the porridges in Mongolia (i.e. semolina) and Cambodia (i.e. rice) were based on chemical analysis in our laboratory (Anderson et?al. 2008a; Lander et?al. 2010). Furthermore, the reliability of the EAR cut-point method for estimating the prevalence of inadequate intakes is reduced when levels of inadequacy are zero or 100% (IOM 2003). Finally, application of the selleck chemical WHO/FAO's (2006) guidelines for fortification also assumes that the diets of the children post-fortification remain unchanged, which may not be the case, especially if the fortified food is expensive, when it may be diluted, or perhaps displace the consumption of indigenous nutrient-dense foods. Among these Asian children, we observed a very high prevalence of inadequate intakes for calcium and iron, but not for zinc, despite the high prevalence of low serum zinc concentrations. This lack of concordance suggests the need for better defined cut-offs for risk of zinc deficiency in children based on the prevalence of inadequate intakes of dietary zinc and/or low serum zinc concentrations. Our estimate for mTOR inhibitor calcium (but not iron) fortificant levels (expressed as mg per 100?g or per daily ration size) to achieve a desirably low prevalence of inadequacy (i.e.