The Next Guide To TRIB1

The second group Akt inhibitor of animals (Cd-treated) was exposed to 20 mg/kg body weight Cd(CH3COO)2 �� 2H2O in drinking (distilled) water once daily for two weeks. During the following 14 days of the experiment, the animals from this group received distilled water and food ad libitum. The third group (monensin-treated mice) was administrated Cd(CH3COO)2 �� 2H2O as described above, followed by treatment with tetraethylammonium salt of monensic acid (16 mg/kg body weight in distilled water) during the days 15 to 28 of the experiment. On day 29 of the experimental protocol, all animals were sacrificed under light ether anesthesia and the samples were collected for analysis. The organs were stored at �C20 ��C prior to analysis. Blood samples were collected in heparinized tubes, centrifuged, and the resulting plasma samples were stored at �C20 ��C. The animal studies were approved by the Ethics Committee of the Institute of Experimental Morphology, Pathology and Anthropology with Museum, Ibrutinib BAS. Chemicals The sodium salt of monensin was a gift by Biovet Ltd. (Peshtera, Bulgaria). Tetraethylammonium hydroxide (Et4NOH), nitric acid (HNO3), and diethyl ether (Et2O) were purchased from Merck (Darmstadt, Germany). Preparation of monensic acid Monensic acid A monohydrate was prepared from sodium monensin (711 mg, 1 mmol) by applying the procedure previously described (Ivanova et al., 2010). Biochemical analysis The biochemical analysis was performed in the clinical laboratory ��Ramus�� (Sofia, Bulgaria) using established analytical protocols. The laboratory ��Ramus�� is certified by the Ministry of Health (Sofia, Bulgaria) to perform clinical analyses. Atomic absorption analysis The organs were digested with concentrated HNO3 (free of metal ions), as described previously (Ivanova et al., 2012). The determination of Cd in the kidneys was performed by a flame (Perkin Elmer Analyst 400, air�Cacetylene flame) atomic absorption spectrophotometer. The analysis of Cd in the hearts of the animals was conducted on an electrothermal analyzer (Zeeman Perkin Elmer 3030, HGA TRIB1 600). Certified Reference Materials from the International Atomic Energy Agency (IAEA-H-8 (kidney) and IAEA-H-4 (animal muscle) were applied to control analytical accuracy. Statistical calculations The results for the three study groups are presented as the mean value �� SD (n=6 for each group). Student's t-test was applied to determine the significance of the differences between the experimental results of two groups. The difference between two groups was considered significant at p