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Версія від 05:53, 16 червня 2017, створена Knot32gallon (обговореннявнесок) (Створена сторінка: monocytogenes growth; with a conclusion that the antimicrobial effectiveness of nisin depends very strongly on the mode of delivery. Incorporating bacteriocins...)

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monocytogenes growth; with a conclusion that the antimicrobial effectiveness of nisin depends very strongly on the mode of delivery. Incorporating bacteriocins into food packaging films to control spoilage of food pathogenic microorganisms has been an area of research for the last decades. Antimicrobial packaging in films prevents microbial growth on the food surface by direct contact of the packaging material with the surface of foods. This is the reason for which the antimicrobial packaging film should ensure its contact with food surface leading to diffusion of bacteriocins to its surface. The controlled release of bacteriocins from food packaging film toward the food surface has a good advantage over dipping, spraying foods with bacteriocins. Antimicrobial activity is lost or decreased because of inactivation of the bacteriocins by components of food or their considerable dilution below active concentration after their migration into the foods (Appendini and Hotchkiss, 2002). Two procedure shave been commonly used to make packaging films with bacteriocins. Two packaging methods, heat-press, and casting, were used to deliver nisin into films made from soy protein and corn zein protein. Both the procedures produced excellent films by inhibiting the growth of the microbe L. plantarum. When the same levels of nisin were incorporated cast films exhibited larger inhibitory zones than the heat-press films. It was also shown that when EDTA was incorporated into the films increased the inhibitory effect of nisin against the bacteria Escherichia coli. Edible cellulosic films made with nisin formulations using HPMC were also produced (Coma et al., 2001). Controlled release application in case of bacteriocins offers a treatment strategy for resistant bacterial strain (Benoit et al., 1998; Chi-Zhang et al., 2004). Research has elucidated that the instantaneous release of nisin can help inhibit microbial cell growth and consequently the survivors will undergo mutations developing resistance to nisin. Also merely releasing nisin from packaging without adding directly to the formulations was not capable of reducing microbial cell counts. A combination of these two resulted both in reduced cell counts as well as a lack of mutation but instead the cells regained their sensitivity to nisin following one transfer passage through nisin-free medium (Chi-Zhang et al., 2004). Nisin- coated polymeric films like PVC, nylon, and linear low- density polyethylene (LLDPE), have found out to be effective in inhibiting Salmonella typhimurium particularly on fresh broiler drumstick skin (Natrajan and Sheldon, 2000). LDPE films which were coated with a mixture of polyamide resin prepared in n-propanol along with a Androgen Receptor Antagonist bacteriocin solution showed an antimicrobial activity against Micrococcus flavus. It was also showed that the efficiency of Nisin coated over LDPE film caused inhibition of M.