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The clinical oral exam was performed by a single, previously trained dentist. This professional verified the presence of oral lesions related to the denture and its condition, to meet the above-mentioned inclusion and exclusion criteria. After the mentioned exams and evaluations, 16 elderly subjects were included in this experiment, and were randomly divided into 2 experimental groups (n=8): Group 1: Adapted toothbrushes handles and Group 2: Toothbrush without adaptation �C conventional type. Fabrication of toothbrush handle devices The devices for the patients of Group 1 were fabricated using heat-polymerized acrylic resin, after having obtained the silicone mold in the shape in which the brush would be held by the participant, [Table/Fig-1]. The technique for fabricating the device was the same as that described by de Mattos et al., [9] [Table/Fig-2]. [Table/Fig-1]: Molding of patient��s hand with condensation silicone [Table/Fig-2]: Finalized toothbrush adaptation device Application of the Experiment The patient��s dentures were evaluated at three time intervals: A �C In the beginning, B - After 7 days and C - After 21 days of using the toothbrush handles. The two groups were instructed to conventional denture hygiene and received new toothbrushes for denture cleaning. For comparative evaluation of the quantity of biofilm, the method used was staining, which is most commonly mentioned in the literature [14]. The revealer used on the internal surfaces of the denture bases was 5% erythrosine, as it has good staining capacity and is easily removed [15]. After the staining procedures, the dentures were placed on a platform at an angle of 45��, with the bases facing upwards, in order to take the photographs with a digital camera (Canon Rebel XT), in a standardized manner. Focus was centered on the median palatal raphe, halfway between the maxillary labial frenum and the posterior base of the denture [2]. The use of photographs contributed Glafenine towards the limitation of the visual method, with low reproducibility and subjectivity [16]. The digital images obtained were analysed with Image Tool? software, by means of which the total area of the internal surface of the denture base and area covered by biofilm were calculated [Table/Fig-3]. The results were expressed in percentage of stained area in relation to the total area of the internal surface of the denture base. [Table/Fig-3]: Software used for biofilm quantification, result expressed in % of area covered by biofilm in relation to total area of the internal surface of the denture base Statistical Analysis All analyses were performed with Biostat version 5.0 software, using Friedman��s test (Non-Parametric Repeated Measures Comparisons), p