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If TAAR glomeruli are part of the DI projection, then TAAR-deletion alleles should diffusely label the entire DI domain, as is seen for class I OR deletions (Bozza et?al., 2009). To test this, we generated three gene-targeted mouse strains with TAAR coding-sequence deletions: ��T4-YFP, in which the Taar4 coding sequence is replaced with Venus YFP; ��T4-RFP, in which the Taar4 coding sequence is replaced with gap-cherry; and ��T9-CFP, in which the Taar9 coding sequence is replaced with Cerulean CFP ( Figure?3A). Strikingly, OSNs expressing these TAAR-deletion alleles densely innervate a small cluster of glomeruli in the dorsal bulb ( Figures 3B and 3C). The cluster comprised 19 �� 3 glomeruli Thalidomide (mean �� SD; n?= 8 bulbs) in heterozygous ��T4-YFP mice. This pattern is in stark contrast to class?I and class II OR deletions, which exhibit sparse labeling of a large number of glomeruli ( Figure?2B; Bozza et?al., 2009). When crossed to the class I and class II markers, the ��T4- and ��T9-labeled glomeruli were distinct from DI and DII glomeruli ( Figures 3D�C3G). Thus, the TAAR-deletion alleles reveal another dorsal projection, named here as DIII, that appears distinct from DI and DII. The DI and DII projections are generated by two types of OSNs (d1 and d2) that selectively express class I or class II genes, respectively (Bozza et?al., 2009). The dense labeling of relatively few glomeruli by ��T4-OSNs is consistent with the idea that they?also exhibit biased coexpression from a limited repertoire of OR?genes��perhaps the TAARs. To test this, we quantified coexpression in ��T4-YFP OSNs by combined immunohistochemistry PD-1/PD-L1 inhibitor 2 and in?situ hybridization, using equally sized class-specific probe mixes (Figures 4A and 4B). Indeed, ��T4-YFP OSNs were much more likely to be labeled by the TAAR mix than the class I or class II mixes (TAAR?= 37%, 47/128 cells; class I?= 0.3%, 1/313 cells; class II: 0/320 selleck kinase inhibitor cells; Fisher's exact test, p?