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All groups were matched for age; male:female ratio; gestation duration; birth weight; maternal age, education and SES. A bootstrap resampling approach (Efron, 1979) with 5000 resamples was used to estimate the distribution of �� and �� for each group, at each voxel, and an unpaired t-test was used (http://fsl.fmrib.ox.ac.uk/fsl/fslwiki/Randomise) to compare �� between each combination of the three groups. Significance was defined as p?BYL719 order ( Worsley et al., 1999). Contiguous clusters were first identified using a threshold of p??3.1). To visually compare the developmental trajectories, areas with significant differences were identified and divided into areas where breastfed?>?formula-fed, breastfed??breast milk and formula-fed, breastfed??breast milk and formula-fed and formula-fed?Pramipexole weight; gestation duration; or maternal age, education or SES. This older age range was used given the similarity in each group's developmental profiles below 800?days. Mean VFM values were compared between the: 1. Breastfed and formula-fed children; and 2. Breastfed and breast milk plus formula children. Prior to comparisons, all data were smoothed with a conservative 2.5?mm full-width-at-half-maximum (FWHM) Gaussian kernel and unpaired SKI-606 datasheet t-tests performed at each voxel to identify significant differences. Significance was defined as p?