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Responses were recorded for 90?min after tetanization and measured as field-excitatory-post-synaptic potential (fEPSP) slope expressed as percentage of baseline. Basal synaptic transmission (BST) was assayed by plotting of the stimulating voltage (V) to slopes of fEPSP to generate input-output relations. The results were expressed as mean �� standard error mean (SEM). For all experiments mice were coded to ��blind�� investigators with respect to genotype and treatment. The results of MWM and open-field test were analyzed using one-way ANOVA and comparison t test. The results of EPM and LTP measurement were analyzed using two-way ANOVA and comparison t test. All other data were expressed as mean �� SEM. The results learn more of Ca2+ leak assay were analyzed by one-way ANOVA with Bonferroni correction. The sample size (��n�� in each group) for each experiment is stated in the figure captions. Minimum statistically significant differences were established at p?find more were prepared from cortex from experimental mice. 100?��g of lysates were separated by PAGE and subjected to Western blots using antibody directed against c-Fos and diluted in LiCOR blocking Megestrol Acetate buffer at 1:1,000 (Cell Signaling, Danvers, MA). Hippocampal lysates were used in protein kinase A (PKA) activity assays (Millipore, Billerica, MA) according to manufacturer's instructions. Briefly, 100?��g of hippocampal lysate was mixed with PKA substrate (kemptide) and [��-32P] ATP in assay dilution buffer in the presence of PKC and CaMKII inhibitors. Samples were incubated at room temperature for 10?min. Reactions were transferred to P81 phosphocellulose squares, which were fixed, washed and transferred to scintillation vials. The 32P incorporated into the substrate was quantitatively measured by its binding to the phosphocellelose paper. The experimental procedure for ER-localized Ca2+ ATPase (SERCA) activity assay was as described previously (Shan et?al., 2010a). The reaction was started by the addition of 100?��g of microsomes to 150?��l of reaction mixture [20?mM MOPS/Tris-HCl, pH 6.8. 100?mM KCl, 5?mM MgCl2, 5?mM ATP, 1?mM EGTA, 0.350?mM CaCl2 (free Ca2+ concentration of approximately 500?nM as calculated using the CHELATOR program).