The Modern Day Guidance On Pazopanib

In the test day rats were removed from their home cages and placed in the activity-monitoring chamber for habituation for 30?min. Following this period, animals received i.p. injections of vehicle (NaCl I-BET-762 in vitro 0.9%) or caffeine at doses of 3, 10, 30, 60 or 120?mg/kg. Immediately after injections, animals returned to the activity-monitoring chamber. Locomotor activity was recorded for 60?min after the injections (n?=?7�C12 per group). The time period of analysis was selected in previous experiments showing that caffeine locomotor stimulation occurred mainly within 60?min. Based on results of experiment 1, caffeine at doses of 30, 60 and 120?mg/kg were chosen to evaluate possible differences of the locomotor depressant drug effect between adolescents and adult rats. To this end, caffeine effect on locomotor activity was evaluated in a protocol in http://www.selleckchem.com/products/Pazopanib-Hydrochloride.html which control rats display high exploratory behavior. This protocol was based on previous data from our laboratory showing that non-habituated rats exhibit an intense locomotor exploratory behavior in the first 10?min of exposure to the novel environment. Also, rats were exposed to the test environment 15?min after vehicle or caffeine injection to ensure the observation of maximum drug effect during the short period of behavioral measure. Then, in the test day adolescent or adult rats (same age described in experiment 1) were transferred to a room adjacent to the behavioral test room and were kept in their home cages for at least 1?h. Next, animals received i.p. injections of vehicle, caffeine Fluvoxamine 30, 60 or 120?mg/kg and were placed in individual cages. Fifteen minutes after injections rats were placed in the activity-monitoring chamber and their locomotor activity was recorded during a session of 10?min (n?=?9�C13 per group). Data were analyzed by Statistica program (StatSoft Inc, Tulsa, OK, USA). In experiment 1, locomotor activity during the 60-min session was analyzed by two-way ANOVA considering the factors age (adolescent and adult rats) and treatment (vehicle or caffeine 3, 10, 30, 60 and 120?mg/kg). In experiment 2, locomotor activity was analyzed by two-way ANOVA considering the factors age (adolescent and adult rats) and treatment (vehicle or caffeine 30, 60 and 120?mg/kg). When significant interaction between factors was detected (P?