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The overall regression model for cognitive empathy was significant; R2=0.33, F(7126)=10.16, pGamma-secretase inhibitor levels of affective empathy. In line with our hypotheses, CU traits were independently negatively associated with levels learn more of affective empathy. Moreover, the CU traits��ASD symptoms interaction effect was marginally significant (p=0.054). This interaction effect was dismantled by examining whether the slopes of the regression lines at low (?1 S.D.), medium (M), and high (+ 1 S.D.) values of ASD symptoms differ significantly from zero ( Cohen et al., 2003). Regression equations were used to plot mean values for affective empathy at minimum (i.e., 100% of raters agreed that child was low CU) and maximum (i.e., 100% raters classified child as high CU) observed values for CU traits as a function of the three levels of ASD symptoms. Fig. 1 illustrates the simple slopes of the regression lines at the three levels of ASD symptoms. There was a significant negative relationship between CU traits and affective empathy at high (��=?0.43, pNK cell at low levels (��=?0.04, p=0.77) of ASD symptoms. In post-hoc analyses we examined whether the abovementioned findings remain significant when controlling for potential confounds associated with child diagnostic comorbidity. Thus, we repeated the analyses above including ADHD and mood disorder symptom severity (as rated by clinicians on the DISCAP) in step 1 of the regression models. There were significant bivariate correlations between ADHD severity and cognitive (r=?0.21, p=0.01) and affective (r=?0.23, p