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In the Lower Tapaj��s River Basin (State of Par��, Brazil), there are approximately 50 communities of diverse sizes and origins, with varying access to health care, education and consumer goods. For this study, we selected 12 communities (Fig. 1) to reflect the diversity of regional populations, social conditions and ecosystems. We examined a large number of outcomes (visual functions, motor performance, and cardiovascular responses) and sample-size calculation, based on visual outcome measurements from preliminary studies, indicated that we would require a minimum of 400 people to test combined effects of Hg and Se on this outcome. Since it is difficult to apply Selleckchem Protease Inhibitor Library a random sampling strategy in this setting (Passos et al., 2007), recruitment was based on a convenience sampling procedure. Several weeks before the present study, each village was visited and the study was explained at village meetings and home visits. Persons 15 years and older were invited to participate on a voluntary basis. The study protocol was approved by the Ethics Review Boards of the University of Quebec at Montreal and the Faculty of Pharmaceutical Sciences of the University of S?o Paulo-Ribeir?o Preto. All participants signed an informed consent form, which was read to them. There was no remuneration for study participation. Participation was limited to a maximum of 12 persons per day BGJ398 mouse because the examination procedure required bringing participants by boat to a technical school in a nearby city, Itaituba, where we had access to electricity and freezers for storing biological material. Each village was scheduled for a specific number of days. The boats arrived the previous evening and made the trip during the night. The study was carried out in two periods from Unoprostone May to July 2006 (May 12 to June 3, and July 5 to July 29). A total of 448 persons (216 men and 232 women) agreed to participate. Reasons for non-participation included work and/or domestic responsibilities, religious convictions, illnesses and lack of interest in the study. We compared participants�� age distribution to the underlying population based on a house-to-house survey carried out in 2003. The participants in the present study represent 25% of the adult population of these villages (27% of all women and 23% of all men). The proportion of persons from each village varied from 10% to 67%, with higher relative frequencies from the smaller villages (the smallest consisted of 13 adult residents and the largest, 384 adults). Younger persons (��15y and