Anti Yeast Infection Diet

Merged photos (C, F) show each proteins co-localised at the apical cell membrane of superficial urothelial cells in wildtype mice (arrowheads, C). In homozygous K7 knockout mice, K18 expression appears to be lowered (E) but remains restricted towards the superficial cell layer within the absence of K7 (E and F). Wildtype (G-I) and homozygous K7 knockout mice (J-L) bladder cryosections double-labelled with antibodies to K7 (G, J) and K20 (H, K). Merged pictures are shown in I and L. Within the bladder of wildtype mice, K20 can also be restricted for the superficial urothelial cells (H) and merged pictures of G and H shows colocalisation with K7 at the apical cell membrane (arrowheads, I). In homozygous K7 knockout mice, K20 expression (K) appeared equivalent to wildtype mice (merged image L). Cryosections have been purchase SB856553 counterstained with DAPI. * indicates the lumen with the bladder and m denotes the position from the underlying bladder mucosa. Scale bars = 50 mm. (TIF) Figure S3 Western blots of basic keratin expression inside the colon and lung of K7 knockout mice. A. Coomassie Blue stained SDS-PAGE gel and B. western blots of cytoskeletal extracts from the colon and lung of wildtype (+/+), heterozygous (+/2) and homozygous (? K7 knockout mice probed with antibodies to K8, K18, K19 and K20. K20 expression was not detected in cytoskeletal extracts in the lung (not shown). M denotes molecular weight requirements, sizes in kDa are as indicated. (TIF) Figure S4 K18 expression within the kidney of homozygous K7 knockout mice. Double-label immunofluorescence microscopy of kidney cryosections from wildtype (A, C, E) and homozygous K7 knockout mice (B, D, F) stained using a rabbit polyclonal antibody to K7 (A, B) and mouse monoclonal antibody Ks18.04 to K18 (C, D). Merged images of A and C and B and D and are shown in panels E and F respectively. In wildtype kidney, both K7 and K18 co-localise and show strong membranous staining of ductal epithelial cells (arrowheads, E). In homozygous K7 knockout mice, the intensity of K18 staining is overall weaker (D) than wildtype kidney (C) while some membranous staining can nevertheless be detected (arrowhead, F). Cell nuclei are counterstained with DAPI. Scale bar = 50 mm. (TIF) Figure S5 K7 and K19 expression inside the liver of K7 knockout mice. Double-label immunofluorescence microscopyTissue Bladder Liver Colon Kidney Lung Pancreas Duodenum StomachK7 expression Urothelium Bile ducts Basal cells in crypts, goblet cells Collecting tubules ductsK8 = = = =KKK20 = ne. = ne. ne. ne. = ="reduced* = = = decreased = = = =" = = = = = = ="Alveolar bronchiolar = epithelium Ductal epithelial cells Brunner's gland distinct cells in crypt = =Squamo-columnar cells = "= intensity of staining and localization equivalent to wildtype tissue. *confirmation by western blotting. ne. no protein expression. " glandular cell staining. doi:10.1371/journal.pone.0064404.tK7 Knockout Miceof liver cryosections from wildtype (A, C, E) and homozygous K7 knockout mice (B, D, F) stained with a rabbit polyclonal antibody to K7 (A, B) and rat monoclonal antibody Troma III to K19 (C, D). Merged images of A and C and B and D and are shown in panels E and F respectively. In wildtype mice, K7 and K19 colocalise and specifically stain the bile duct epithelium (E). Within the liver of homozygous K7 knockout mice, K19 staining is just not altered by the absence of K7 (D, F).