HENSIVE Technique Method. Cianzio, S.R.

Nevertheless, molecular tools applied to both biological entities, soybean and nematodes, have opened up possibilities for escalating breeding efficiency. This presentation will D. In this experiment, we determined the minimum {number address the present use of technologies. It'll also address how the advent of new molecular info and technologies may well further contribute to enhance breeding efficiency in soybean for resistance for the soybean cyst nematode. NEW SPECIES OF CALOOSIA, CRICONEMA, HEMICYCLIOPHORA AND MESOCRICONEMA (NEMATODA: CRICONEMATOIDEA). Cordero, Marco and R.T. Robbins. Plant Pathology Department, 2601 N. Young Ave. CralleyWarren Study Lab. University of Arkansas, Fayetteville, AR. 72704. New species of Criconematoidea identified in Arkansas and North Carolina among 2008-2011 are reported. Nematodes were killed and fixed in hot three formaldehyde, and subsequently infiltrated with glycerin working with Seinhorst's modified slow approach. Morphological characterization was performed by acquiring the nematode's morphometrics represented by measurements and ratios utilizing light microscopy (Nikon Optophot-2) along with a Nikon Drawing Tube. Caloosia n. sp. was identified linked with pines and Paspalum sp. is characterized by having a lengthy body (1081.3-1431.three mm), long stylet (105.6-123.8 mm), flattened physique annuli (R=338-357), interruptions in body annuli laterally that demarcate a single lateral field without having lines along with a basic rounded vulva slit (V=75.8-77.eight), without modified vulva lips. Criconema n. sp. 1 was identified linked with hackberry, Paspalum sp., maple and oatgrass. The species has a conspicuous head offset in the body with two annuli, brief roundedMeeting Abstracts 459 tail having a thin cuticular sheath and subterminal anus. Criconema n. sp. two was located associated with Paspalum sp. Specimens have two cephalic annuli regarding the exact same width, 1st annulus directed posteriorly, separated by a narrow neck annulus and a quick conoid tail using a unilobed non-folded annulus. Hemicycliophora n. sp. was found related with turfgrass in North Carolina and is characterized by having a lateral field demarked by two faint lines with transverse anastomoses and/or breaks with the striae, extended vulva lips and vulval sleeve, an elongated, non offset, conical tail, with distinct annulations ending within a rounded tip. Mesocriconema n. sp. was identified connected with Paspalum spp. and is characterized by getting little flattened submedian lobes, reduce than labial disc or at the identical level, vagina straight, extremely effectively created rounded spermatheca devoid of sperm, no far more than one anastomoses, L= 379-512 mm, V=88.7-92.9, stylet length = 48.7-60.9 mm, R=107-119, annuli with crenate margins at tail portion plus a easy anterior vulva lip. ITS-1 amplicons have been obtained and submitted to GenBank. The genus Caloosia has been reported in India, Bangladesh, Sri Lanka, South Africa, Fiji, and Hem in the population (Keinan and Clark 2012). Exome sequencing Hawaii islands. That is the very first report of your genus in North America. PREPARE YOURSE.HENSIVE Program Strategy. Cianzio, S.R. Raymond F. Baker Center for Plant Breeding, Iowa State University, Ames, IA. Among the pathogenic species of nematodes threatening soybean (Glycine max) production, the soybean cyst nematode (Heterodera glycines; SCN) may be the most damaging. The release of cultivars resistant to SCN is consequently of utmost value, considering that genetic resistance is definitely the most efficient mode to manage the pathogen and protect soybean yield.