N Fig. 15B); tarsal claw relatively significant and stout with a

Basic coloration orangish yellow shaded extensively margin of femur densely covered with thick setae, and N Fig. 15B); tarsal claw reasonably huge and stout having a posterior margin roundly expanded at apex bearing a group of stout acute spines (Fig. Crucial for the species of Asthenopus Male 1 ?Penile lobe (distad to basal thumb) with a equivalent width along its length, basal thumb separated by a wide furrow (Figs 17A , E ); fnhum.2013.00686 forceps very stout (ratio length/ basal width = four.7?.0) ...........................................................2 Penile lobe (distad to basal thumb) wider basally, basal thumb fused to penile lobe (Fig. 17D,G); forceps comparatively slender (ratio length/ basal width = six.2?.0) ......................................................................................................Phylogeny and biogeography of Asthenopodinae using a revision of Asthenopus...Figure 16. Asthenopus fore (FW) and hind wings (HW) of male imago. A A. curtus FW HW C A. magnus FW HW E A. hubbardi FW HW G A. guarani, FW (specifics) HW J A. angelae (from Argentina) FW HW.N Fig. 15B); tarsal claw relatively huge and stout having a row of marginal denticles (Fig. 15G). Leg II (Fig. 15C): smaller sized, with thinner femora, with scattered extended setae, mostly basally and along hind margin; tibia and tarsi with row of long setae on outer (dorsal) margin, ventrally with quite a few stout spines on apical half, using a distal brush of thick setae (arrow in Fig. 15C); tarsal claw comparatively modest, without the need of denticles. Leg III (Fig. 15D, F): as leg II except larger and with anterior margin of femur densely covered with thick setae, and posterior margin roundly expanded at apex bearing a group of stout acute spines (Fig. 15F); tibia without the need of distal brush. Coxae I and II directed ventrally, coxae III directed laterally. Abdomen. Gill I decreased in size, double, both portions subequal in length and width. Gills II II nicely developed, ventral portion smaller than dorsal portion; tergum X with well developed ventral spine on posterior margin (not visible dorsally, Fig. 15E). Caudal filaments brief (curved in mature nymphs) with whorls of stout spines and uncomplicated setae at joinings. Distribution. Amazonas and Parana biogeographic subregions (Argentina, Bolivia, Brazil, Colombia, Ecuador, Peru). Discussion. The genus Asthenopus has been distinguished by indicates from the following characters (Dom guez 1988): 1) ratio foreleg/FW male: 3/5?/5; two) male foretarsus 2.5 times longer than foretibia; three) foretarsal segment 2 related for the other individuals, and 2/3 the length of tibia); 4) ratio length of Rs stem/fork to margin 1/4 (or fork Rs at 2/10 from base to margin) ; five) cubital intercalaries slightly diverging toward hind margin, ICu2 and ICu1 basally fused to CuA by cross veins; 6) ICu2 ending at anal margin or in the tornus, 7) marginal intercalary veins absent; eight) forceps ratio width/length: 1/7; eight) penes robust on basal 2/3; 9) MA fork base to margin 10/100; 11) IMP P1 not fused basally; 12) MP2-IMP comparable in length, not fused; 13) foretarsal claws of male not so expanded distally (as in Asthenopodes). Our phylogenetic analyses only recovered some of these character states as synapomorphies of this genus (see diagnosis and Appendix 2). The proposal of fnins.2013.00251 Dom guez (1988) and Hubbard Dom guez (1988) concerning the intermediacy of Priasthenopus gilliesi with respect to Asthenopus curtus and Asthenopodes picteti is in concordance with our final results. Priasthenopus gilliesi resulted sister for the Povilla-Asthenopus clade, presenting some plesiomorphic character states shared with Asthenopodes.