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The following laboratory investigations were done: complete blood count CBC with differential count, C-reactive protein (CRP), liver function tests [total and direct bilirubin, international normalized ratio (INR), and serum albumin], liver enzymes [alanin aminotransferase (ALT), aspartate aminotransferase (AST)]. The adrenal function of all patients was assessed by low dose test (LDT) through measuring the basal level of cortisol at 10?am; then a 1?��g of synthetic ACTH analogue through IV access was given for stimulation of the adrenal gland, after 30?min a second blood sample was obtained for estimation of increment of cortisol level [10]. A Basal cortisol levels?S1PR1 relative adrenal insufficiency was considered if the increment in cortisol level was?Bleomycin manufacturer Excel 2003 software package. Data were presented in the form of mean?��?SD. Categorical data were presented in the form of number and percentage. Comparisons between various studied groups were performed by using Mann Whitney U test or t-test. Associations between categorical parameters were performed by using chi square test (��2 value) or Fisher��s exact test. Power of significance (probability): P?>?0.05 is not significant. P?Selleck Rigosertib cysts (n?=?2), gastroenteritis (n?=?2), and spontaneous bacterial peritonitis (n?=?1). Blood culture was done for 7 patients and revealed no growth in 3/7, Klebsiella in 2/7, and Staphylococcus aureus in 1/7 and Escherichia coli in 1/7. Patients received intravenous antibiotics either according to culture and sensitivity or broad spectrum antibiotics. Albumin and diuretics were given if indicated in addition to other supportive measures. Children of group 2 had liver cirrhosis due to biliary atresia in 7/12, progressive familial intrahepatic cholestasis in 3/12, and 2/12 with cytomegalo viral hepatitis (CMV). Most of them scored as Child�CPugh A (40.7%). No patient of group 1 or group 2 had absolute adrenal insufficiency (Table 1).