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Ovarian longitudinal (d1) and anteroposterior diameters (d2) were measured in the ovaries' maximum longitudinal section; ovarian area was calculated according to the following formula: Area (cm2)=0.785(��/4)��d1��d2[15]. Ethical consideration This study was approved by the Ethics Review Committee of the First Affiliated Hospital of Xi'an Jiaotong University and informed consent with detailed descriptions of the Fleroxacin potential benefits and risks of the study was obtained from all participants. Statistics All data were entered into Microsoft Excel 2007 (Microsoft Corporation; Redmond, WA, USA), and all statistical analyses were done in the software SPSS version 11.5 (SPSS Inc.; Chicago, IL, USA). Differences of means of the measurement data were tested for statistical significance with Student's t test, with repeated ANOVA test, while chi-square test was employed to compare the difference of proportions. Optimal cut-off value of endometrial thickness was explored by ROC analysis and AUC score. A P-value RO4929097 solubility dmso Results General characteristics During the follow-up period, pathologic examinations were performed on endometrial specimens collected from subjects with abnormal uterine bleeding. There were 49 in the treatment group and 37 from the control group. Two hundred fifty-two and 281 subjects from the treatment and control group were enrolled in TVS monitoring (Fig. http://www.selleckchem.com/products/NVP-AUY922.html 1). There were no significant differences between the treatment group and control group in age, age at menopause, duration of menopause, age at menarche and body mass index (all P>0.05, Table 1). Fig. 1 Flow diagram of the progress of the study. Table 1 Baseline characteristics of the study participants (mean�� SD). Ovarian area changes The average ovarian area of patients in the treatment group and the control group is shown in Table 2. There was no statistical difference, between the treatment group and the control group, in the average ovarian area at different time periods (all P>0.05). Table 2 Comparison of ovarian area in the treatment and control group (cm2). Changes in uterine volume The mean uterine volume of the subjects receiving tibolone did not significantly differ from that measured before treatment (P>0.05). However, there were significant differences in the size of uterine volume between before and after treatment in the control group (P