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?2, B, C, F, G, J, and K). Fluorescence intensity LY294002 at fissure sites appeared to be only marginally stronger than deeper within the tissue. Fluorescence intensity deep within the tissue was similar between uninjured, injured, and sliced explants. For injured and sliced explants, mean fluorescence intensity within the first 50 ��m depth from the surface in contact with the bath was normalized to that of uninjured explants ( Fig.?2, D, H, and L). Statistical comparisons were made for all intact and fissured surfaces within injured and sliced explants to the surface of uninjured explants. For all solutes, these surface fluorescence intensities were not significantly different between intact surfaces of injured or sliced explants and surfaces of uninjured explants with the exception of the intact surface of injured explants exposed S6 Kinase to FITC, where the normalized mean intensity was 18% (p?check details sliced explants exposed to FITC were significantly higher than fluorescence intensities of surfaces of uninjured explants, having normalized mean intensities of 316% (p?