Make Your Daily Life Simpler Thanks to ARAF Understanding

Financial support was provided in part by the Banack Family Partnership Endowed Teaching Chair in Agriculture to P.P. Footnotes Citation Fillmer K, Adkins S, Pongam P, D��Elia T. 2015. Complete genome sequence of tomato mosaic virus isolated from jasmine in the United States. Genome Announc 3(4):e00706-15. doi:10.1128/genomeA.00706-15.""Microvirga vignae strain BR 3299T was isolated from a cowpea root nodule growing in a soil sample collected from a Brazilian semiarid region (1). Most bacteria isolated from cowpea nodules cultivated in this region are not taxonomically characterized, although Pictilisib price they are potentially members of Microvirga (2, 3). Drought stress is one of the major harsh environmental conditions affecting rhizobium symbiosis in semiarid lands. Furthermore, soil temperatures near the surface can be very high, and although they decrease rapidly with increasing depth, being around 30 to 35��C at 15?cm, the negative impact on legume nodule formation and dinitrogen fixation can be significant for several species (4). Here, we report the draft genome sequence of strain BR 3299, a type strain of M.?vignae and a symbiotic nitrogen-fixing member of the Alphaproteobacteria. BR 3299T total genomic DNA was extracted and purified (5) from a culture grown in yeast mannitol agar (YMA) medium. The whole genome was sequenced using the 100-bp paired-end Illumina MiSeq system (Macrogen, South Korea), yielding a total of 11,703,138 reads, which corresponds Ceritinib clinical trial to approximately 181�� coverage sequencing. The read quality was analyzed using FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc) and trimmed using the FASTX-Toolkit, and only bases with a quality score of >20 (Q20) were used. De novo assembly was performed using CLC Genomics Workbench 7 (CLC bio, USA), and contigs of ARAF sequence were carried out using the RAST version 2.0 server (6). tRNAs were predicted with tRNAscan-SE 1.23 (7) and rRNAs were predicted with RNAmmer 1.2 (8). The KEGG pathways and Pfam annotation were analyzed using WebMGA (9). The annotated genome was submitted to the Joint Genomics Institute (IMG/ER database) (https://img.jgi.doe.gov/cgi-bin/er/main.cgi) which was used to refine the annotation and registration at the International Nucleotide Sequence Database Collaboration (GenBank, USA). The draft genome sequence of M. vignae strain BR 3299T consists of 165 contigs and includes 6,472,445?bp, with an overall G+C content of 61%. In the genome, 6,641 protein-coding sequences (CDSs), distributed in 451 subsystems, as well as 60 copies of RNA were predicted. Approximately 62.7% of the CDSs were assigned specific functions using RAST annotation, and a Pfam reference family was identified for 36% of the CDSs, whereas KEGG orthology (KO) numbers were assigned for 65%. All enzymes required for biological nitrogen fixation are encoded by the genome.