Ree Quick Life (PTSL) entails the

Early investigation showed that Ors is relatively uninformative, and overlaps {with rootstock affected PTSL incidence, and a collaboration of Clemson University and USDA-ARS cause the release of a much more tolerant rootstock trademarked Guardian`BY520-9'. To investigate the genetic and molecular basis for this tolerance, Nemaguard, a PTSL susceptible rootstock, and Guardianselection 3-17-7 had been crossed. The F1 plants were self pollinated to create segregating F2 populations. The trees have been rated annually, from 2004 by means of 2008, for their response to PTSL. One hundred and seventy-six microsatellite/Simple Sequence Repeat (SSR) markers, every mapping to a distinct chromosomal location around the Prunus reference nuclear genome, have been applied to screen the two parents and F1-11. Only 53 markers showed polymorphism amongst the parents, and were heterozygous in F1-11. These SSRs were then screened on the F2-11 population (N=100). Segregation information for PTSL-response and SSR marker inheritance were complied and subjected to Analysis of Variance to establish differences in Genotypic Implies (GMs). Nine SSR loci correlated having a response to PTSL. These nine SSR loci had been distributed on 4 linkage groups (e.g., Linkage Group-1, LG-2, LG-4 and LG-6). 4 of the nine loci (EPDCU5100, Pacita 27, UDA008 and UDA029) accounted for considerably on the variance inside the trait primarily based upon their significant GMs (>2.5). Immediately after creating a molecular map from the segregation information, QTL evaluation identified a quantitative locus on LG2 that was critical in all five years from the study. Additionally, this chromosomal interval contained three in the four SSR marker loci that accounted for the majority of your variation. These results identified regions with the peach genome exactly where genes controlling PTSL susceptibility and tolerance reside, as a result helping to define the genetic basis for response to PTSL as a complex but tractable trait. Disease resistant genes found within the area with the most promising PTSL-associated QTL is going to be discussed. HOST Range ADDITIONS FOR HETERODERA URTICAE AND CACTODERA BETULAE FROM ARKANSAS. Robbins1, Robert T., and Weimin Ye2. 1Plant Pathology Dept., University of Arkansas, Fayetteville, AR 72701; and 2North Carolina Department of Agriculture Customer Services, Nematode Assay Section, 4300 Reedy Creek Road, Raleigh, NC 27607-6465. In January, 2012, samples were taken from many different winter weeds at Toad Suck Park around the Perry County bank of the Arkansas River near Conway, Arkansas. Cysts of Heterodera urticae, that was first reported in the 2011 SON meetings, had been present in substantial numbers. Essential inspection of these plant roots revealed white females attached for the roots of typical chickweed (Stellaria media (L.) Vill. Numerous juveniles were identified in the soil related with chickweed, but males were not present. Many species of winter weeds had been inoculated with juveniles obtained from cysts recovered in the soil to decide other probable hosts. Final results of this test are nonetheless to become determined. A practical difficulty that could take place with H. urticae on weeds in soybean fields may be mis-identification a.Ree Brief Life (PTSL) involves the interaction of environmental conditions (winter temperature, soil history, early pruning) and pathogens ring nematode (Mesocriconema xenoplax) and Pseudomonas syringae.Meeting Abstracts 487 PTSL is characterized by premature tree death by bacterial canker or cold injury, resulting in substantial financial losses, which have been observed in the Southeastern U.S.