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The data presented in this study learn more show that the VC is capable of providing rapid filovirus quantitation from seed stocks with results that significantly correlate with the plaque assay (r = 0.9984, p SUDV and MARV are presented. While no direct correlation between concentrations of the various virus stocks were noted, the particle counts were greater than the plaque titers but lower than the genomic equivalents. Variability seen is possibly due to the time or amount of cell lysis when each preparation was harvested or the multiplicity of infection (MOI) used to produce the stocks, all of which are known to affect the infectious virus check details titer. Kikwit preps 1 and 2 represent similar Vero E-6 passage three stocks prepared at an MOI of 0.01, while prep 3 and 4 represent passage two stocks of the same EBOV variant. Prep 3 was produced at an MOI of 0.01 and prep 4, like the SUDV Gulu and MARV Angola variants, were produced at an MOI of 0.001. Results reveal that there is about a log surplus of viral genetic material produced when compared to the number of virus particles as measured by the VC and an additional two logs more particles than infectious virus. These results may be due to the amount of cell lysis at the time of harvest or perhaps inefficient filovirus virion packaging or both. Similar patterns were noted for filoviruses by Weidman and colleagues when comparing plaque assay, Autophagy qRT-PCR, and a focus-forming assay using virus-specific antibodies for a variety of hemorrhagic fever viruses [17]. Their study demonstrated that some viruses, for example Lassa and yellow fever, were more efficient at replication and packaging of infectious viruses than others such as filoviruses and Rift Valley fever virus. As shown in Table 1, the VC and TEM particle counts were within one log of each other, which is also within the normal variability seen for the TEM (data not shown). As TEM improvements are instituted and variability of this technique is improved this difference should decrease. The VC assay is simple to conduct, training is minimal, and no virus-specific reagents are required. Due to the use of an instrument for analysis, quantification is non-subjective and requires