The 4-Minute Technique For S6 Kinase

05; Figure ?Figure6A).6A). On days 3 and 7 of therapy, the number of PCNA-positive cells in the kidneys of fKSC treated rats were significantly higher as compared to those in the saline treated rats (P S6 Kinase nick-end labeling (TUNEL)-positive cells in kidney sections of saline and fetal kidney stem cells (fKSC) treated ... Administration of fKSC promotes renal angiogenesis On day 3 of therapy, the morphometric analysis of CD31 labeled peritubular capillaries showed a significantly higher capillary density in the kidneys of fKSC treated than that in saline treated rats (13.30 �� 1.54 vs 7.10 �� 1.29, capillaries/HPF, P find more Figure 8 Early angiogenic effect of administered fetal kidney stem cells in cisplatin injured kidney. Representative immunoblots showing the expression of hypoxia-inducible factor (HIF)-1��, vascular endothelial growth factor (VEGF) and endothelial nitric ... DISCUSSION The present study demonstrates that fKSC expressed mesenchymal as well as renal progenitor markers and exhibited the formation of CD31 and vWF expressing capillary-like structures selleck screening library and differentiation into CK18 and CK19 positive epithelial cells in vitro. The administration of fKSC in rats with cisplatin induced ARF resulted in rapid improvement in renal function and histology. The infused fKSC were observed to engraft in renal tubules and promote proliferation and reduce apoptosis of renal tubular cells. In addition, the kidneys of fKSC treated rats exhibited increased angiogenesis and up-regulation of angiogenic signaling molecules. To our knowledge this is the first study showing therapeutic efficacy of in vitro expanded fKSC in cisplatin induced ARF model and role of angiogenesis in renal regeneration by these stem cells. We have recently observed that fKSC have maximal growth at a seeding density of 1000 cells/cm2, population doubling time of approximately 34 h and normal karyotype up to 3rd passage.