The Beneficial, Unhealthy And also Alizarin

Colocalization of the AhR and ARNT within the nucleus was seen in the lower epidermal area. In the control group, the AhR Alizarin and ARNT were weakly stained in the epidermis and there was no colocalization of the two molecules (Fig. S1). The expression levels of the AhR, ARNT and CYP1A1 mRNA were higher in the AD group than in the control group (Fig. S2a). The quantities of mRNAs of ARNT (P?Fulvestrant solubility dmso difference among the three groups (detailed results are in the supplementary data, Fig.?1). We analysed the cytokine expression patterns of NHEKs with ELISA after treatment with TCDD or PCBs at various concentrations. IL-6 and IL-8 showed a dose-dependent increase after treatment with both TCDD and PCBs. Both cytokines reached their peak levels 4 h after treatment with PCBs and 8 h after treatment with TCDD. The levels of IL-6 and IL-8 were higher after treatment with TCDD than after treatment with PCBs. We also examined the antagonistic effect of the AhR. After additional 10?nmol/l of CH223191 treatment, both IL-6 and IL-8 production was reduced (Fig.?2a�Cd). To verify whether the change in cytokine expression pattern was due to the activation of the AhR signalling pathway, we localized the AhR and ARNT in NHEK after treatment with 10?nmol/l of TCDD and PCBs and observed the internalization of AhR to the nucleus. When NHEK was treated with TCDD, the AhR showed high signal intensity in the nucleus at three and 6 h after the treatment and did the ARNT. On the other hand, when SB203580 cell line treated with PCBs, the AhR and ARNT were colocalized in the nucleus after 24?h. Both the AhR and ARNT were diffusely and weakly stained throughout the entire cell when treated with DMSO only (Figs S3 and S4). In conclusion, the results of this study suggest that the AhR is highly expressed in the acute lesional skin of patients with AD and psoriasis and that activation of the AhR increases the expression of inflammatory cytokines in NHEK. As this is only a pilot study, we are planning on further studies with increased sample size, and additional studies such as immunoblot analysis, to investigate the role of AhR. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (No.2011-0013003, 2012R1A1B3002196, 2011-0012878) and the Research Grant of Kwangwoon University in 2013.